Mechanisms of Sequence Dependent Translational Stalling
نویسندگان
چکیده
bundles undergo deformations highly similar to a twisted rod under compression. Our data suggests that tau-like proteins minimize microtubule lattice interactions and the prevent torque generation that leads to extreme neuron deformations. These experimental results, together with mechanical modeling of the neuron, suggest that spectrin tension and microtubule bundle mechanics are crucial for stabilizing chiral cytoskeletal networks and produce a specialized cell shape that we propose is critical for neuronal function.
منابع مشابه
Ribosome occupancy of the yeast CPA1 upstream open reading frame termination codon modulates nonsense-mediated mRNA decay.
Saccharomyces cerevisiae CPA1 mRNA contains an upstream open reading frame (uORF) encoding the arginine attenuator peptide (AAP). Negative translational regulation of CPA1 occurs when the nascent AAP responds to arginine (Arg) by stalling ribosomes at the uORF termination codon. CPA1 expression is also controlled by nonsense-mediated mRNA decay (NMD). Using wild-type and decay-defective strains...
متن کاملCritical 23S rRNA interactions for macrolide-dependent ribosome stalling on the ErmCL nascent peptide chain
The nascent peptide exit tunnel has recently been identified as a functional region of ribosomes contributing to translation regulation and co-translational protein folding. Inducible expression of the erm resistance genes depends on ribosome stalling at specific codons of an upstream open reading frame in the presence of an exit tunnel-bound macrolide antibiotic. The molecular basis for this t...
متن کاملModeling translation elongation dynamics by deep learning reveals new insights into the landscape of ribosome stalling
Translation elongation plays a central role in multiple aspects of protein biogenesis, e.g., differential expression, cotranslational folding and secretion. However, our current understanding on the regulatory mechanisms underlying translation elongation dynamics and the functional roles of ribosome stalling in protein synthesis still remains largely limited. Here, we present a deep learning-ba...
متن کاملStructural basis for translational stalling by human cytomegalovirus and fungal arginine attenuator peptide.
Specific regulatory nascent chains establish direct interactions with the ribosomal tunnel, leading to translational stalling. Despite a wealth of biochemical data, structural insight into the mechanism of translational stalling in eukaryotes is still lacking. Here we use cryo-electron microscopy to visualize eukaryotic ribosomes stalled during the translation of two diverse regulatory peptides...
متن کاملNot4-dependent translational repression is important for cellular protein homeostasis in yeast.
Translation of aberrant or problematic mRNAs can cause ribosome stalling which leads to the production of truncated or defective proteins. Therefore, cells evolved cotranslational quality control mechanisms that eliminate these transcripts and target arrested nascent polypeptides for proteasomal degradation. Here we show that Not4, which is part of the multifunctional Ccr4-Not complex in yeast,...
متن کاملTranslational attenuation and mRNA stabilization as mechanisms of erm(B) induction by erythromycin.
Translational attenuation has been proposed to be the mechanism by which the erm(B) gene is induced. Here, we report genetic and biochemical evidence, obtained by using erythromycin as the inducing antibiotic, that supports this hypothesis. We also show that erythromycin increases the level of the erm(B) transcript by stalling the ribosome on the leader mRNA and thereby facilitating the stabili...
متن کامل